The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-10　　PDF

Structural analysis of AFGP by MALDI-TOF MS

(Hokkaido Univ.)
oKohki Fukushi, Hiromu Fukasu, Shogo Urakami, Hiroshi Hinou

Antifreeze glycoprotein (AFGP) was discovered in Antarctic fish as a substance that lowers the freezing point of blood. Throughout our study on the synthesis of natural-type(linear) and cyclic AFGPs, we showed that cyclic AFGPs also possess antifreeze activity. In contrast to linear AFGPs, Cyclic AFGPs with shorter peptide chains showed higher antifreeze activity, despite having the same tripeptide unit with disaccharide.
In this study, we report the results of Matrix-assisted laser desorption/ionization–time-of-flight (MALDI-TOF) and TOF/TOF mass spectrometry (MS) analyses of linear and cyclic antifreeze glycoproteins in order to promote structure-activity relationship studies of AFGPs.