Symposium Sessions (Day1, Day2, Day3)
Basic Sessions (Day1, Day2, Day3)
Young Researchers' Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 2, May 16（Tue.） Room P (Foyer, Room 1004-1007)
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2P-09 PDF
MIMICS: In Vitro Co-Culture Device for Investigating Host-Microbe Interaction via an Integrated multiomics analysis
Akkermansia muciniphila has been identified as a potential next-generation probiotic that could enhance host health and prevent metabolic diseases. However, the biological interactions between A. muciniphila and human intestinal epithelial cells (Caco-2) for use in biotherapeutics have not been extensively studied. To address this knowledge gap, we developed an in vitro culture device named MIMICS, which simulates the human intestinal environment and enables multi-omics analysis. Our study demonstrated that both A. muciniphila and human intestinal epithelial cells remained viable for 12 hours after co-culture in MIMICS. Subsequently, we conducted transcriptomic and proteomic analyses to observe the changes in intestinal epithelial cells caused by A. muciniphila treatment, including changes in cell-cell junctions, immune response, and mucus secretion. Our findings are consistent with those reported in previous in vivo studies. Furthermore, our results revealed that A. muciniphila activates glucose and lipid metabolism in intestinal epithelial cells, leading to an increase in ATP production. Overall, our study suggests that A. muciniphila can activate metabolism in intestinal epithelial cells and that the developed MIMICS system is a useful tool for evaluating the effects of bacteria on intestinal epithelial cells in vitro.