The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-07　　PDF

Application of Targeted Proteomics by nano-LC-MS/MS to Lipomyces starkeyi

(1Osaka Univ., 2Niigata Univ. Pharm. Appl. Sci.)
oTakafumi Iwakura1, Taisuke Seike1, Nobuyuki Okahashi1, Rikako Sato2, Hiroaki Takaku2, Fumio Matsuda1

Targeted proteomics using nano-LC-MS/MS was applied to the oleaginous yeast Lipomyces starkeyi. The protein extraction method was optimized by using a multi beads homogenizer. MRM assay methods including 2,288 transitions were also constructed to measure the expression levels of 107 enzyme proteins. The targeted proteomic analysis of the central metabolism-related enzymes revealed that those expression levels in the lipid production phase were significantly lower than that of the exponential growth phase.