The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-06　　PDF

Towards Comprehensive Detection of Protein Conformational Change by Limited Digestion

(1Kyoto Univ., 2NIBIOHN)
oYuka Muramatsu1, Kosuke Ogata1, Yasushi Ishihama1,2

Proteins are biological elements that regulate cellular functions, and their higher-order structures are closely related to their functions. Proteome-scale structural analysis of proteins is essential to elucidate the molecular basis of the ability of cells to change their phenotype in response to external environmental stimuli. One promising method is Limited Proteolysis-coupled Mass Spectrometry (LiP-MS), which combines limited protein digestion with mass spectrometry. However, the protocol is not yet mature enough, and experimental conditions for effective exploration of proteome-scale structural changes need to be investigated. Therefore, in this study, optimization studies of various experimental conditions, including partial digestion, were conducted to comprehensively detect structural changes related to protein function.