Symposium Sessions (Day1, Day2, Day3)
Basic Sessions (Day1, Day2, Day3)
Young Researchers' Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 2, May 16（Tue.） Room P (Foyer, Room 1004-1007)
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2P-05 PDF
Investigation of the Relationship between Virulence Factors, Antibiotic Resistance, and SarA in Methicillin-Resistant Staphylococcus aureus (MRSA) using DNA Affinity Capture Assays
In this study, we investigated the mechanisms of antibiotic resistance in Methicillin-resistant Staphylococcus aureus (MRSA), a bacterium known for developing resistance to many antibiotics. We used a DNA affinity capture assay (DACA) to identify the global regulator, which controls various virulence factors and regulatory proteins contributing to antibiotic resistance. The mecA, sarA, and sarR promoters were used, which have significant effects on MRSA survival. The results showed that SarA binds to all three promoters, indicating its essential role in regulating these promoters. Furthermore, we analyzed a mutant strain that lacked SarA and found that it had reduced antibiotic resistance and biofilm formation. Furthermore, comparative proteomics study was conducted using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analysis to identify differences in protein expression between the SarA mutant and the wild-type. The data showed that SarA positively regulates the agr system, which promotes phenol-soluble modulins transcription and inhibits Rot translation, while negatively regulating extracellular proteases and PBP2a expression, which affects biofilm formation and antibiotic resistance, respectively. In addition, the SarA sequence was highly conserved across clinical strains from different lineages, indicating its potential as a crucial target for developing therapeutics against MRSA infections.