The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-01　　PDF

A novel matrix alkylated hydroxy chalcone for peptides analysis by MALDI-MS

(1Hiroshima Univ., 2Shimadzu)
oManaho Yamaguchi1, Yuko Fukuyama2, Koichi Tanaka2, Syunsuke Izumi1

In matrix assisted laser desorption/ionization mass spectrometry, matrices more suitable for analytes are selected. Matrices such as alkylated trihydroxy acetophenone (ATHAP) with alkyl chains are suitable for hydrophobic peptide analysis 1). In this study, alkylated hydroxy chalcone (AHC), a compound with alkyl chains like ATHAP, was used as a matrix, and hydrophobic peptide humanin was detected as [M+H]+ from outer edges of a matrix/analyte crystal spot. However, the poor crystallinity of AHC may reduce reproducibility. In order to overcome this weakness, AHC was mixed with 2, 5-dihydroxy benzoic acid (2, 5-DHB). In addition, AHC with a high absorbance at 355 nm was expected to improve the sensitivity. As a result, when a 9:1 (v/v) mixture of 2, 5-DHB and AHC was used as a matrix, hydrophobic peptides ions were detected with higher sensitivity.