The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Young Researchers' Sessions

Day 2, May 16（Tue.）　10:30-10:45　Room C (Room 1009)

2C-O-1030（2P-22）　　PDF

Development of 13C-Metabolic Flux Analysis for HL-60 Neutrophil-like Cell Using GC-MS

(Osaka Univ.)
oTakeo Taniguchi, Nobuyuki Okahashi, Fumio Matsuda

13C-metablic flux analysis using mass spectrometry was employed to investigate metabolic flux distributions in undifferentiated, differentiated, and LPS-activated HL-60 myeloid leukemia cells. First, the metabolic model was determined by 13C-tracing. Glucose and glutamine, as well as aspartate, and glutamate were incorporated into cells. The metabolic flux distribution calculated using the constructed metabolic model showed that the level of TCA cycle fluxes was constant, while differentiated HL-60 decreased the level of glycolysis flux compared to that of undifferentiated HL-60. LPS-activated differentiated HL-60 cells showed that increased levels of flux of glucose uptake, pentose phosphate pathway and NADPH regeneration.