The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Young Researchers' Sessions

Day 2, May 16（Tue.）　09:45-10:00　Room C (Room 1009)

2C-O-0945（2P-18）　　PDF

One-step enrichment of protein terminal peptides using pipette tip columns for protein terminomics

(1Kyoto Univ., 2RIKEN IMS, 3NIBIOHN)
oKazuya Morikawa1, Hiroshi Nishida1, Koshi Imami1,2, Yasushi Ishihama1,3

Comprehensive profiling of protein termini is important for understanding the function of proteoforms. In this study, we developed a new protocol for the CHAMP-N method, a one-step enrichment of protein N-terminal peptides by strong cation exchange chromatography (SCX) for protein digested peptides by LysargiNase. In this new protocol, a disposable pipette-tip SCX microcolumn is used instead of a conventional HPLC column, allowing for simplified, parallel processing and high-throughput analysis of protein N-terminal peptides. Furthermore, by optimizing the elution conditions, more protein N-terminal peptides were successfully identified than in the conventional HPLC-based method. In this presentation, we will also report the results of comprehensive analysis of protein N- and C-terminal peptides using our recently developed C-terminal peptide enrichment method, CHAMP-C, combined with the CHAMP-N method.