The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan

Abstract

Basic Sessions

Day 2, May 16(Tue.) 09:15-09:33 Room B (Room 1202)

Data processing methods for simultaneous hybrid imaging by organic and inorganic mass spectrometers

(Univ. Tokyo)
oToshihiro Suzuki, Hui Khoo, Takafumi Hirata

The laser ablation system was connected to two mass spectrometers in parallel, and simultaneous hybrid imaging was performed. A portion of the aerosols were transferred to an in-house dielectric barrier discharge ionization source connected to an organic mass spectrometer for molecular analysis, while the other portion of the aerosols were transferred to an ICP-MS for elemental analysis. A green coffee bean was used as the sample for imaging analysis.
Since transport time of aerosol streams to each mass spectrometer is different, adjustment of horizontal position is inevitable in hybrid imaging technique. We developed new software, BioQuant, for hybrid imaging analysis. An overlapping image of organic-MS and ICP-MS is displayed by BioQuant, and adjustment of image position can be performed by mouse operation.
The horizontal size of unit cell of image is approximately 53μm in ICP-MS image while it is around 48μm in organic-MS image. Hence, a new time span was defined as the new unit cell of image, and signal intensities were re-calculated with this time interval. By using this BioQuant for data analysis, the distribution of metal ions and biomolecules can be visualized and correlated.