The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Symposium Sessions

Day 2, May 16（Tue.）　15:27-15:45　Room A (Special Conference Room)

2A-S-1527　　PDF

Simultaneous analysis of polar metabolome and lipidome by unified-hydrophilic interaction/anion-exchange liquid chromatography tandem mass spectrometry (unified-HILIC/AEX/MS/MS)

(Kyushu Univ.)
oKohta Nakatani, Kazuki Ikeda, Masatomo Takahashi, Takeshi Bamba, Yoshihiro Izumi

Metabolomics is a study of a complete set of metabolites including hydrophilic and hydrophobic metabolites in biological samples. However, an ideal analytical method that can simultaneously analyze the polar metabolome and lipidome has not been developed because of the extremely wide range of their physicochemical properties. We have previously reported a unified hydrophilic-interaction/anion-exchange liquid chromatography tandem mass spectrometry (unified-HILIC/AEX/MS/MS) for polar metabolome analysis (Nakatani et al., </I>, 2022) and a lipid class separation-based method using HILIC for lipidome analysis (Takeda et al., </I>, 2018). Here, we report an update of the unified-HILIC/AEX/MS/MS method, which integrates lipidome analysis into the HILIC-dominant phase of the unified-HILIC/AEX separation, allowing simultaneous analysis of polar metabolome and lipidome.