The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Symposium Sessions

Day 2, May 16（Tue.）　14:51-15:09　Room A (Special Conference Room)

2A-S-1451　　PDF

Development of Organelle Metabolomics by Live Single-cell Mass Spectrometry

(1Meijo Univ., 2Univ. Shizuoka, 3Yokogawa Electric, 4ITO EN)
oHajime Mizuno1,2, Yuta Terui3, Hironori Takai3, Aogu Furusho2, Eiji Sugiyama2, Kenichiro Todoroki2, Iwao Sakane4

There are increasing demands to study the molecular and cellular behavior of a single cell precisely because the current knowledge in life science was obtained using multiple numbers of cells with little information on localization. We developed “Live Single-cell Mass Spectrometry” technique which allows direct analysis of xenobiotics and endogenous compounds in a living cell. The technique is comprised of two steps; 1. capturing of cellular content from a living cell using a metal coated glass microcapillary under video-microscopic observation, and 2. the capillaries are directly subjected to static nanoelectrospray ionization of the sampled cellular content such as cytosol or organelle using mass spectrometry. Currently, to improve the sensitivity and precision of this method, we are studying the development of an automated sampling system, improvement of data variability due to matrix effects, and improvement of mass spectrometry sensitivity by derivatization.