The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-41　　PDF

Serum Metabolite Analysis and Principal Component Analysis Using Liquid Chromatography Ion Mobility Mass Spectrometry

(1Toyama Pref. Univ., 2Toyama Pref. Univ., 3Kanazawa Univ.)
oRiko Takata1, Mai Yamakawa2, Ryosuke Koseki1, Shigehiro Karashima3, Issey Osaka1,2

In quantitative analysis of steroids using LC/MS/MS, multiple ion monitoring (MRM) method has been used for highly sensitive detection. The MRM is targeted analysis method. Therefore, unknown and unexpected components could not be detected by MRM. Comprehensive analysis is required to elucidate various disease in metabolomics. In this study, metabolites in the serum were detected with high sensitivity and selectivity using LC/IM/MS. Steroids ions for targeted analysis and lipid ions for non-targeted analysis were observed. In addition, obtained LC/IM/MS data were statistically analyzed by principal component analysis (PCA). Healthy subjects and various hypertensive patients were classified based on steroidal differences.