The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-39　　PDF

Development of metabolic rewiring analysis method using metabolomics data and flux data

(Osaka Univ.)
oMasaharu Maruyama, Nobuyuki Okahashi, Fumio Matsuda

A novel method to identify metabolic regulatory reactions during metabolic rewiring was developed by integrating the data sets of metabolomics and 13C-metabolic flux analysis and employed for the human breast cancer cell line under treatment with paclitaxel, an antimitotic agent. The substrate accumulation against flux decreases of phosphofructokinase (PFK) and pyruvate kinase (PYK) indicated that the fluxes were mainly controlled by enzyme activity. This method was useful for identifying metabolic regulatory reactions, because PFK and PYK, which were known to regulate glycolysis, were identified to be regulated after paclitaxel treatment.