The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-37　　PDF

A Method for Efficient and Accurate Identification of Proteins of Malaria Parasites (Plasmodium falciparum) and Human Prepared from Infected Red Blood Cells by using Mass Spectrometry

(1Kobe Univ., 2Kobe Univ., 3Kobe Univ., 4Kobe Pharm. Univ.)
oKen-ichi Yoshino1,2, Ayaka Omoda3, Konatsu Okada3, Atsuko Takeuchi4, Hideyuki Iriko3

There are only 413 entries of Plasmodium falciparum proteins in the SwissProt (uniport_sprot.fasta, October 2022 edition)．Therefore, sequence database searches against the SwissProt database using mass spectrometry data from samples prepared from infected human erythrocytes containing malaria parasite P. falciparum-derived proteins may not identify significant proteins if the major component is a malaria parasite P. falciparum-derived protein. In addition, “false positive” results of identifying human proteins that are not major components may arise. In this presentation, we report a method for efficiently and accurately identifying P. falciparum-derived proteins in samples containing both P. falciparum-derived and human proteins.