The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-24　　PDF

Searching of temperature-sensing regions of Adenylate kinase 1 by H/D exchange with MALDI-MS

(Suntory Foundation for Life Sciences)
oTatsuya Yamamoto, Honoo Satake

Adenylate kinase 1 (AK1) is a highly structural-conserved enzyme involved in energy metabolism and catalyzes the phosphoryl exchange reaction ATP + AMP ↔ 2ADP. The activities of AK1s are controlled by temperature, and the optimum temperature for activity appears to be proportional to body temperature. To elucidate common law of AK1 activities from many species, we analyzed Searching of temperature-sensing regions of AK1 from E. coli, C. Intestinalis, X. laevis, G. gallus, H. Sapiens by MALDI based-H/D exchange mass spectrometry (HDX-MS) and the calculation software “Scipas DX”