The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 1, May 15（Mon.）　　Room P (Foyer, Room 1004-1007)

1P-17　　PDF

Electron Activated Dissociation-Based MS/MS Platform to Elucidate Plant Specialized Metabolite Structures

(1Tokyo Univ. Agr.Tech., 2RIKEN CSRS)
oYoshimasa Todoroki1, Yuki Matsuzawa1, Kazuki Saito2, Tetsuya Mori2, Hiroshi Tsugawa1,2

The comprehensive profiling of plant specialized metabolites (natural products) by liquid chromatography coupled with tandem mass spectrometry is an outstanding issue in metabolomics due to the lack of authentic standard compounds. Therefore, we evaluated the tandem mass spectra (MS/MS) of electron activated dissociation (EAD) to elucidate the natural product structures. The EAD-MS/MS spectra of 716 standard compounds were obtained, and the spectral entropy distribution was investigated. The spectra were further evaluated for predicting metabolite classes in molecular spectrum networking, where we found that more informative product ions were obtained in EAD-MS/MS to elucidate the plant metabolites including alkaloids.