The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Symposium Sessions

Day 1, May 15（Mon.）　14:38-15:00　Room A (Special Conference Room)

1A-S-1438　　PDF

The practice of epitope mapping against a glycoprotein by the latest HDX-MS system

(Shionogi)
oOta Takeshi, Masahiro Takayama, Hiroshi Ueda

Recently, it has been reported that epitope mapping of monoclonal antibody against hemagglutin was performed by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS), while glycosylation sites were uncovered due to the difficulty of detecting and quanitfying peptides containg glycans by HDX-MS1). Here, we will introduce an approach for epitope mapping on glycosylated SARS-CoV2 receptor-binding domain of S protein by the latest HDX-MS system consist of Orbitrap Eclipse, PAL and UPLC.
1) Puchades C, et al. Sci Rep. 2019 Mar 18;9(1):4735.