The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Symposium Sessions

Day 1, May 15（Mon.）　14:15-14:38　Room A (Special Conference Room)

1A-S-1415　　PDF

Immunoreactivity Profiling of Anti-Chinese Hamster Ovarian Host Cell Protein Antibodies by Isobaric Labeled Affinity Purification-Mass Spectrometry Reveals Low-Recovery Proteins

(1Kyoto Univ., 2Daiichi-Sankyo, 3NIBIOHN)
oShunsuke Takagi1,2, Masayoshi Shibata2, Nobuyuki Suzuki2, Yasushi Ishihama1,3

We evaluated the immunoreactivity profiles of eight commercial anti-host cell protein (anti-HCP) antibodies from different host animals and their antigens used for immunization by newly developed isobaric labeled affinity purification-mass spectrometry (AP-MS) method. As a result, 34 proteins with high abundance but low recovery from harvest cell culture fluid were identified. Since they are likely to be underestimated in biopharmaceutical quality assessment, the features common to these proteins were investigated. Compared to other immunoprecipitated HCP proteins, proteins exhibiting lower molecular weight (ΔMW = −14600), lower isoelectric point (ΔpI = −0.86), and lower hydrophobicity (ΔGRAVY = −0.13) were enriched. This AP-MS method provides important information for HCP control strategies using immunological methods and is expected to contribute to the development of safe biopharmaceutics.