演題概要

ポスター発表

第3日　5月13日（水）　　P会場（1008/09）

3P-24　　PDF

ESIにおける脱溶媒温度変化に伴うスペクトル変化を利用した分子質量推定法

(¹エムエス・ソリューションズ・ ²浜松医大・ ³プレッパーズ)
^o高橋豊^1,2,3

Electrospray ionization　(ESI), the most general ionization method in LC/MS, is a representative example of soft ionization, and usually does not generate fragment ions. However, in some compounds which have a hydroxyl group, [M+H]+, [M+Na]+, [M+NH4]+, etc. are not generated by positive ions, and dehydration from [M+H]+ and [M+H-H2O]+ are mainly observed. If such ions are observed in the measurement of an unknown sample, it will cause inconvenience when acquiring mass information of the analyte. In this study, in the ESI used for LC/MS, the dehydration ions [M+H-H2O]+ from [M+H]+ are mainly observed at normal desolvation temperatures. This paper reports a method for obtaining mass information of an analyte correctly from changes in mass spectral patterns by lowering the desolvation temperature. The performance was demonstrated using cholesterol, eudesmol and hinesol.