演題概要

ポスター発表

第3日　5月13日（水）　　P会場（1008/09）

3P-17　　PDF

サイクリックイオンモビリティータンデム質量分析による誘導体化した単糖、二糖の構造解析

(¹ジョージア工科大学・ ²Waters - Pleasanton・ ³Waters - Wilmslow・ ⁴日本ウォーターズ)
Mckenna, Kristin¹・ Baker, Andrew²・ Palmer, Martin³・ Cooper-shepherd, Dale³・ Fernandez, Facundo¹・ ^o窪田雅之⁴

Small sugars represent an interesting class of molecules that are difficult to characterize using conventional ion mobility spectrometry (IMS). By adding a suitable covalent shift reagent, differences in collisional cross section (CCS) are amplified, allowing for better identification and characterization. 3-carboxy-5-nitrophenylboronic acid (3C5NBA) reacts with hydroxyl groups on the sugars to form bidentate derivatives depending on the available cis-diol reaction sites defined by the sugar composition, carbohydrate linkage, and conformation. High resolution IMS using a variable path length mobility separator and tandem-IMS strategies (further separation of mobility-selected sub-populations) can be used to probe the complex mixture of isomeric carbohydrate derivatives.