演題概要

ポスター発表

第3日　5月13日（水）　　P会場（1008/09）

3P-02　　PDF

細胞内分子観察に向けたエレクトロスプレー支援レーザー脱離イオン源の開発

(¹阪大工・ ²阪大MEIセンター・ ³島津・ ⁴阪大院生命機能)
^o三好悠斗¹・ 閑念弘樹^1,3・ 間久直¹・ 粟津邦男^1,2,4

Imaging of intracellular molecules is important analytical technique to understand dynamics of proteins and metabolites in a cell. In previous study, using mass spectrometry imaging, high spatial resolution (1 μm) was achieved. However, sensitivity of ions is not sufficient. In order to acquire clear and detailed image, it’s necessary to increase amount of ions. In this experiment, to improve ionization efficiency, ELDI (Electrospray-assisted laser desorption/ionization; ELDI) source combining laser desorption ionization (LDI) and electrospray ionization (ESI) under atmospheric pressure and optical system was constructed. Using ELDI, crystal violet (CV) was measured and the effectiveness of ELDI source was evaluated.