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ポスター発表

第2日　5月12日（火）　　P会場（1008/09）

2P-24　　PDF

質量分析法を用いた骨髄性白血病細胞株HL-60の¹³C代謝フラックス解析

(阪大院情報)
^o谷口赳夫・ 岡橋伸幸・ 松田史生

The ¹³C-metablic flux analysis using mass spectrometry was used to investigate metabolic flux distributions in differentiated and non-differentiated HL-60 myeloid leukemia cells. A time course analysis of cell numbers, medium components and ¹³C-labeling kinetics of the intracellular metabolites confirmed that ¹³C-MFA could be applied for the immune cells. Flux distributions in the central carbon metabolism suggested that differentiated HL-60 cells shifted the metabolism from glucose to amino acids-dependent.