演題概要

ポスター発表

第2日　5月12日（火）　　P会場（1008/09）

2P-18　　PDF

セスキテルペン合成経路解明のためのAspergillus nidulansの揮発性代謝物質の質量分析 VIII. AN3280 遺伝子発現誘導株： LO8925 株および LO8910HA-pyrG2 株

(¹奈良女大院総合科学・ ²名大院医・ ³奈良女大理)
^o服部真理子¹・ 紅朋浩²・ 竹内孝江^1,3

Microbial volatile organic compounds (MVOCs) emitted from Aspergillus nidulans were investigated using Head Space-SPME/GC/MS, and the effects of modified expression of some sesquiterpene biosynthesis genes in the genome of A. nidulans were analyzed. In strain LO8910HA-pyrG2, a part of AN3280 gene upstream was eliminated from LO8910. The eliminated region seemed to surpress transcription in the parental strain LO8910. 7-epi-neopetasone, which is an intermediate of PR-toxin, was detected in LO8910HA-pyrG2 as reported previously in LO8925. We also found that LO8910HA-pyrG2 can produce a new sesquiterpene MVOC.