ポスター発表
- 第2日 5月12日（火） P会場（1008/09）
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2P-14 PDF
LC/MS/MSによる遺伝子組換えヒトラミニン-511E8の部位特異的糖鎖解析
Laminin-511 E8 is a glycoprotein consisting of α5, β1 and γ1 chains. Recombinant laminin-511 E8 is often used for a culture of stem cells as a matrix. Although this protein has ten potential N-glycosylation sites, neither glycosylation site nor glycan structure is still known. We conducted site-specific glycosylation analysis by proteases digestion, glycopeptide-enrichment, and nanoLC/MS/MS. We demonstrated that most glycosylation sites were occupied with fucosyl biantennary glycans.