ポスター発表
- 第2日 5月12日（火） P会場（1008/09）
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2P-09 PDF
ソフトウェア支援による合成オリゴ核酸のMS/MS配列確認
Synthetic oligonucleotides have emerged as promising therapeutic agents for the treatment of a variety of diseases, including viral infections and cancer. HR-MS/MS is widely adopted for the sequence confirmation of peptides, but the application to oligonucleotide has not been succeeded because of the software which enables computed interpretation of MS/MS spectra. We analyzed oligonucleotides that have the same sequence in the 3’-region, and MS/MS confirmations were performed with ion-pairing reversed phase chromatography and QTOF-MS. It is showed that the common fragment ions were detected and annotated automatically by -a, -b, -c, -d, -w, -x, -y, -z ions nomenclature with AOS (Agilent Oligonucleotide Sequencer) software.