演題概要

ポスター発表

第1日　5月11日（月）　　P会場（1008/09）

1P-21　　PDF

GC/MS calibration-curve-locking database (CCLD)を用いたヒト血漿メタボロミクス実験室間検証

(¹アジレント・ ²名大院医・ ³東農大・ ⁴水産研・ ⁵九大生医研)
^o姉川彩¹・ 相澤有美³・ 上山純²・ 辻井良政³・ 羽野健志⁴・ 秦康祐⁵・ 山下俊幸⁵・ 和泉自泰⁵・ 馬場健史⁵

In this study, we performed cross-laboratory validation of gas chromatography/mass spectrometry (GC/MS) with a calibration-curve-locking database (CCLD) to integrate metabolome data obtained at different facilities. The GC/MS-based metabolomic data was compared at five different laboratories using identical plasma samples, pretreatment method, and GC/MS analysis. Results indicated that the concentration of hydrophilic metabolites in human plasma NIST SRM1950 is almost consistent with the certificated values. Thus, these studies suggested the possibility of semi-quantification screening using CCLD without requiring calibration curve.