ポスター発表
- 第3日 5月17日（金） P会場（多目的ホール）
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3P-37 PDF
ハートカット二次元液体クロマトグラフィー（2D-LC法）を用いた，はちみつ中のグルコン酸の炭素安定同位体比分析
In 2004, liquid chromatography combined with isotope ratio mass spectrometry (LC/IRMS) has been developed, which allows the measurement of stable carbon isotope (δ13C) composition of highly polar and non-volatile analyses. Recently, LC/IRMS has been used to authenticate and trace products such as honey, wine, and lemon juice, and compounds such as caffeine and pesticides. However, analysis of complex multicomponent samples is difficult due to limitations of flow rate and mobile phase as a problem of LC/IRMS.
Herein, we present a heart-cutting two-dimensional (2D)-LC/IRMS method with high resolution for complex multicomponent samples. Using the simulated honey sample, the conditions were optimized by the switching time test, the interfering carbohydrate level test, and the detection limit test, and the results were highly accuracy and precision. In this study, we analyzed gluconic acid in honey containing high-carbohydrate-content samples for the first time. These values shed some light on the mechanism of gluconic acid production. We expect this method to find utility for stable isotope analysis of other complex, multicomponent samples containing compounds that interfere with the target compound.