演題概要

ポスター発表

第3日　5月17日（金）　　P会場（多目的ホール）

3P-30　　PDF

CCAP法により合成された部位特異的抗体薬物複合体の特性解析

(¹日本ウォーターズ・ ²鹿児島大院理工・ ³量研)
^o川瀬泰司¹・ 廣瀬賢治¹・ 岸本聡²・ 畠中孝彰²・ 安達基泰³・ 伊東祐二²

To demonstrate liquid chromatography mass spectrometry (LC/MS) characterization of site-specific Antibody-drug conjugate (ADC) synthesized using chemical conjugation by affinity peptide (CCAP). Conjugation was confirmed by UltraPerformance LC (UPLC) equipped with quadrupole time-of-flight (Q-Tof) MS and drug-to-antibody ratio (DAR) of 2 was calculated by mass shift. Conjugate site was estimated by LC/MS peptide mapping using various enzyme digestion.