演題概要

ポスター発表

第3日　5月17日（金）　　P会場（多目的ホール）

3P-25　　PDF

LC/MSを用いたアルドースレダクターゼの基質特異性の分析

(¹武庫女大バイオ研・ ²武庫女大薬)
^o堀山志朱代¹・ 萩中淳^1,2

Since aldose reductases (ARs) have a lot of families, it is important to develop inhibitors against AR families as drug candidates. Therefore, we tried to clarify the substrate specificity of AKR1B10, one of the AR families, and to evaluate the inhibitory activity of epalrestat, which is one of the inhibitors against AKR1B10, using synthesized substrates. It was found that AKR1B10 selectively reduced the aldehyde form of a substrate, not the keto form. This result suggests that the developed method can be applied to the clarification of the substrate specificity of ARs and the screening of inhibitors against ARs.