日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第3日 5月17日(金)  P会場(多目的ホール)

イメージング質量分析法を用いた糖尿病性腎症モデルラット腎の高深度プロテオーム解析

(1同志社大生命医2ブルカージャパン3国循研)
葛原由貴1武笠結天1o韮澤崇2梶田遼2植田初江3角田伸人1池川雅哉1

Diabetic nephropathy (DN) is a major life-threatening complication of diabetes. Renal lesions affect glomeruli and tubules, but the pathogenesis is not completely defined. Here we have tried to generate in depth proteomic analysis of DN using diabetic animal model, a new inbred rat strain, Spontaneously Diabetic Torii (SDT) fatty rats, which develop hyperglycemia with obesity after 17 weeks of age in comparison with normal control Sprague Dawley (SD) rats of the same age. In this study, we applied matrix-assisted laser desorption/ionization (MALDI) mass imaging mass spectrometry (IMS) for the study of pathological aspects of DN, especially generating in depth proteome analysis by integrating MALDI-IMS and shotgun analysis (timsTOF Pro system).