演題概要

ポスター発表

第3日　5月17日（金）　　P会場（多目的ホール）

3P-20　　PDF

ホルマリン固定サンプルを用いたイメージング質量分析法とLC-TIMS-MS/MSの統合ワークフローによる腎アミロイドーシスの病態解明

(¹同志社大生命医・ ²ブルカージャパン・ ³Necker-Enfants Malads Hospital・ ⁴Georges Pompidou European Hospital・ ⁵国循研)
^o武笠結天¹・ 韮澤崇²・ 梶田遼²・ Rabant, Marion³・ Jean-Paul, Huyen³・ Patrick, Bruneval⁴・ 植田初江⁵・ 角田伸人¹・ 池川雅哉¹

Here we have established an integrated workflow of matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) in combination with shotgun proteomics using liquid chromatography, trapped ion mobility and mass spectrometry (LC-TIMS-MS/MS). An analytical technique was developed and evaluated through a combination of specific sample preparation/extraction protocols based on the same formalin-fixed tissue section of renal biopsy tissue samples. Our strategy succeeded in the discrimination of subgroups of renal amyloidosis such as immunoglobulin light chain (AL) and serum amyloid A (SAA) type. With the current strategy, we have detected some of the proteins that may provide insights into the pathogenic process of amyloid formation in human kidneys.