日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第3日 5月17日(金)  P会場(多目的ホール)

MALDI-イメージング質量分析法とショットガンプロテオミクスを用いた拡張型心筋症モデルJ2N-kハムスターの病態解析

(1同志社大生命医2京大院医3ブルカージャパン4国循研)
o新谷祈1辻崇2石田瑞希1韮澤崇3角田伸人1植田初江4升本英利2湊谷謙司2池川雅哉1

Dilated Cardiomyopathy (DCM) has an extremely poor prognosis. To investigate the pathogenesis of DCM, we performed global proteomic analysis of myocardial tissues from J2N-k cardiomyopathic hamsters. In the current study, we tried to integrate in situ proteomics for cardiac tissues from hamsters by integrating Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) and LC-trapped ion mobility spectrometry (TIMS)- MS/MS . Histological examinations as well as serial cardiac tissue sections from hamsters at each weeks of age were analyzed with rapiflex tissue typer and tims TOF Pro with nanoElute. MALDI-IMS in combination with a shotgun proteomic analysis is a useful approach in analyzing cardiomyopathic tissues from DCM animal model.