日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第3日 5月17日(金)  P会場(多目的ホール)

高感度かつ再現性の高い代謝物分布解析を可能にする質量分析イメージング試料調製法の開発

(1九大院農2島津3産総研)
o一瀬智美1藤村由紀1村山布紗1山崎雄三2山本卓志2割石博之1三浦大典3

Matrix-assisted laser desorption ionization (MALDI)-mass spectrometry imaging (MSI) enables to visualize the distribution of biomolecules. The choice of the optimized matrix deposition method is critical for visualizing distribution of biomolecules with high sensitivity and high spatial resolution. Generally, manual or automated spray-coating and automated sublimation methods are used, but these methods have some drawbacks with respect to detection sensitivity, spatial resolution, and data reproducibility. Herein, we present the 9-aminoacridine (9-AA) deposition method of sublimation coupled with recrystallization. The matrix recrystallization process after sublimation was optimized for the solvent concentration and reaction temperature. After sublimation, a 9-AA-coated glass slide was exposed to 5-20% methanol vapor at 30-80°C in a reaction chamber constructed in our laboratory. We found that 5% methanol at 60°C condition can provide clear images of the representative 9-AA-sensitive nucleotide derivatives. Next, we investigated the three different matrix application methods (automated spray-coating, sublimation, and sublimation coupled with recrystallization methods) for their detection sensitivity, spatial resolution and reproducibility. Our optimized method showed the best detection sensitivity and provided clear images for nucleotide derivatives and lipids. In addition, this method provided higher reproducible MSI data with higher spatial resolution than the other two methods.