オーラルセッション
- 第3日 5月17日（金） 16:05～16:25 E会場（201）
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3E-O2-1605 PDF
親水性相互作用/陰イオン交換クロマトグラフィータンデム質量分析による次世代メタボローム分析法の開発
Essential metabolic pathways to maintain biological activities include glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, amino acid, and nucleic acid metabolism. Almost all intermediates of these metabolic pathways are polar and ionic compounds such as amino acids, nucleic acid constituents, organic acids, sugar phosphates, coenzyme, etc. Therefore, a profiling for these hydrophilic metabolites provides valuable insight towards understanding metabolic regulation. However, since these hydrophilic metabolites have a wide variety of physicochemical properties, it is difficult to comprehensively measure them with a single analytical run. In the present study, we have developed a highly sensitive and practical analytical method that provides wide metabolite coverage using mixed-mode hydrophilic interaction/anion exchange liquid chromatography tandem mass spectrometry (HILIC/AEX/MS/MS) with a novel polymer-based amino column.