演題概要

オーラルセッション

第3日　5月17日（金）　15:05～15:25　E会場（201）

3E-O2-1505　　PDF

質量分析を用いたがん代謝の計測ー非上皮性細胞由来がんの代謝フラックス解析

(阪大院情報)
^o島知輝・ 丸山正晴・ 岡橋伸幸・ 松田史生

The ¹³C-metabolic flux analysis (¹³C-MFA) using mass spectrometry was attempted to investigate metabolic flux distributions in the non-epithelial cancer cells including YKG-1 (human glioma) and HT-1080 (human sarcoma). A time course analysis of cell numbers, medium components and ¹³C-labeling kinetics of the intracellular metabolites confirmed that ¹³C-MFA could be applied for the non-epithelial cancer cells. Flux distributions in the central carbon metabolism suggested that there was variation in the NADPH method among the cancer cell lines.