日本質量分析学会 第67回質量分析総合討論会

演題概要

オーラルセッション

第3日 5月17日(金) 16:25~16:45 D会場(202)

ペプチドのlow/high-energy CIDにおけるプロリン効果と分子内水素移動

(1横市大2島津)
o高山光男1神子島朝希1関谷禎規2岩本慎一2田中耕一2

Intramolecular hydrogen transfer to form [y+2H]+ ions formed by the proline effect in low- and high-energy (E) CID experiments are examined using a synthetic peptide EGV-d5 containing deuterated Ala(d3)-Pro and Gly(d2)-Pro residues. The high-E CID resulted in [y]+ and [y+2H]+ ions originated from cleavage at the Xxx-Pro residues, while low-E CID merely resulted in the [y+2H]+ ions. The rates of deuterium (hydrogen) transfer from alpha-carbon (Gly-d2), beta-carbon (Ala-d3) and other sites to form [y+HD]+ and [y+2H]+ are estimated from the isotope patterns of y-related ions. The mechanism for the formation of [b]+, [y]+ and [y+2H]+ ions due to the proline effect also is described in both low- and high-E CID experiments.