オーラルセッション
- 第3日 5月17日(金) 16:25~16:45 D会場(202)
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3D-O2-1625 PDF
ペプチドのlow/high-energy CIDにおけるプロリン効果と分子内水素移動
Intramolecular hydrogen transfer to form [y+2H]+ ions formed by the proline effect in low- and high-energy (E) CID experiments are examined using a synthetic peptide EGV-d5 containing deuterated Ala(d3)-Pro and Gly(d2)-Pro residues. The high-E CID resulted in [y]+ and [y+2H]+ ions originated from cleavage at the Xxx-Pro residues, while low-E CID merely resulted in the [y+2H]+ ions. The rates of deuterium (hydrogen) transfer from alpha-carbon (Gly-d2), beta-carbon (Ala-d3) and other sites to form [y+HD]+ and [y+2H]+ are estimated from the isotope patterns of y-related ions. The mechanism for the formation of [b]+, [y]+ and [y+2H]+ ions due to the proline effect also is described in both low- and high-E CID experiments.