日本質量分析学会 第67回質量分析総合討論会

演題概要

オーラルセッション

第3日 5月17日(金) 15:05~15:25 B会場(102)

イメージング質量分析によるマウス組織中のアンチセンス核酸とその代謝物の検出法の開発

(1大塚製薬2阪大院薬3医薬基盤健栄研)
o横井宏之1,2笠原勇矢3小比賀聡2,3土井健史2鎌田春彦3

Oligonucleotide therapeutics are currently receiving great attention as next-generation pharmaceuticals. But its pharmacokinetic evaluation methods are still not sufficient, and in particular, more tools are needed to evaluate the tissue distribution of administered drugs and their metabolites.
MALDI-IMS was a breakthrough technique for localizing biomolecules directly in tissue sections. The advantage of MALDI-IMS is that it can localize multiple molecules in tissue sections simultaneously and label-free. This technique has been applied to the detection of administered drugs and their metabolites in tissue sections.
In this study we focused on antisense oligonucleotides, and developed a MALDI-IMS-based detection method to clarify the tissue distribution of antisense oligonucleotides and their metabolites. As a model antisense oligonucleotide, we used an antisense oligonucleotide containing locked nucleic acids (LNA-A).
Analysis of LNA-A-treated mouse livers and kidneys were performed using the developed method. In the liver sections, MALDI-IMS revealed that LNA-A was uniformly distributed. In the kidneys sections, MALDI-IMS revealed that LNA-A localized in a portion presumed to be the renal cortex. We also obtained information on LNA-A metabolites, which showed the same distribution profile as LNA-A in livers and kidneys.