演題概要

ポスター発表

第2日　5月16日（木）　　P会場（多目的ホール）

2P-35　　PDF

MALDI - イメージング質量分析法を用いた実験的自己免疫性脳脊髄炎マウス中枢神経系組織プロテオーム解析による多発性硬化症のバイオマーカーと治療標的の特定

(¹同志社大生命医・ ²ブルカージャパン・ ³京府医大・ ⁴関西医大)
^o田中那美¹・ 山下浩輝¹・ 韮澤崇²・ 梶田遼²・ 田口勝敏³・ 田中雅樹³・ 近藤誉之⁴・ 辻雄大¹・ 角田伸人¹・ 池川雅哉¹

Multiple sclerosis (MS) is an autoimmune disease its cardinal symptoms are chronic inflammatory, demyelination and axonal degeneration of central neuro system (CNS). In this study, we applied matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) for　CNS of murine EAE without on-tissue digestion. We have successfully identified and visualized some specific proteins specifically expressed in lesions and investigated a candidate of therapeutic target. It was validated on an EAE model with or without blocking agents of the proteins. Furthermore, using LC-TIMS-MS/MS, in depth proteomics for the cells infiltrated into CNS was also performed.