ポスター発表
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2P-34 PDF
マウス皮膚組織に経皮投与した薬剤のイメージング質量分析を用いた動態解析
The transcutaneous vaccine which is low-invasive, easy-to-use, and safe compared with the conventional injectable vaccine is attracting worldwide attention for the countermeasures of infectious diseases. In order to develop an efficacious transcutaneous vaccine capable of reducing antigen dosage and dose frequency, we need to search and investigate adjuvant candidates that can be used for transdermal administration. In transcutaneous vaccines targeting the skin, a detailed understanding of the localization and kinetics of antigens and adjuvants in skin tissue will provide a theoretical basis for efficacy and safety. The purpose of this study is to clarify the kinetics of imiquimod, an adjuvant candidate for transcutaneous vaccine, in the skin tissue. Therefore, we focused on imaging mass spectrometry that can analyze the intrinsic dynamics of the adjuvant. Skin tissues removed 2, 6, 12 and 24 hours after transdermal administration of imiquimod were measured. As a result, it is supposed that imiquimod remains inside the skin tissue for up to 6 hours after transdermal administration. Imiquimod may be delivered to lymph nodes through lymphatics after 6 hours. It is thought that the maturation of dendritic cells showing antigen presentation to T cells is promoted and finally the adjuvant effect is exerted.