演題概要

ポスター発表

第2日　5月16日（木）　　P会場（多目的ホール）

2P-32　　PDF

LC-QTOF-MSを用いたグラム陰性細菌成分Lipid Aの分析

(¹理研IMS・ ²阪大院情報・ ³慶大薬)
^o岡橋伸幸^1,2・ 安田柊¹・ 津川裕司¹・ 池田和貴¹・ 有田誠^1,3

A method for the analysis of lipid A, an important immune activator of gram-negative bacteria, was developed by using LC-QTOF-MS. The sharp peak of lipid A was observed by employing a metal-free column and EDTA-containing solvent. MS/MS spectra assignment enabled the structure estimation of lipid A. This method was successfully applied to the analysis of cultured Escherichia coli in vitro and fecal sample of wild type mice. The results suggest the structural diversity of lipid A in fecal microbiome.