目次
ポスター発表
- 第2日 5月16日（木） P会場（多目的ホール）
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2P-25 PDF
LC-MS/MSを用いた血清中の抗体薬物複合体（ADC）の定量系の構築 —特性解析からバイオアナリシスまで—
An analytical method for quantifying ADC in serum with LC-MS/MS has been developed using Kadcyla which is commercially available ADC. As a result of measuring an enzymatic digest of Kadcyla using trypsin, V8 and Asp-N using Q-TOF/MS, 32 of 44 sites of lysine in Kadcyla were identified as conjugation sites of DM-1. The conjugation ratio of DM-1 for each lysine was estimated from the peak area ratio of drug conjugate to non-conjugate. Based on these results, a specific payload-linked peptide was selected from the tryptic digest. By using these peptides, a bioanalytical method with LC-MS/MS was constructed.