日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第2日 5月16日(木)  P会場(多目的ホール)

LC-MS/MSを用いた血清中の抗体薬物複合体(ADC)の定量系の構築 —特性解析からバイオアナリシスまで—

(東レリサーチセ)
o太田里子齋藤恵矢崎啓寿櫻井周

An analytical method for quantifying ADC in serum with LC-MS/MS has been developed using Kadcyla which is commercially available ADC. As a result of measuring an enzymatic digest of Kadcyla using trypsin, V8 and Asp-N using Q-TOF/MS, 32 of 44 sites of lysine in Kadcyla were identified as conjugation sites of DM-1. The conjugation ratio of DM-1 for each lysine was estimated from the peak area ratio of drug conjugate to non-conjugate. Based on these results, a specific payload-linked peptide was selected from the tryptic digest. By using these peptides, a bioanalytical method with LC-MS/MS was constructed.