演題概要

オーラルセッション

第2日　5月16日（木）　10:50～11:10　B会場（102）

2B-O1-1050　　PDF

MALDI-イメージング質量分析法とショットガンプロテオミクスを用いたアルツハイマー病脳病理解析の新たな試み

(¹同志社大生命医・ ²ブルカージャパン・ ³都健康長寿医セ研・ ⁴同志社大脳科学)
^o池川雅哉¹・ 角田伸人¹・ 宮坂知宏¹・ 韮澤崇²・ 梶田遼²・ 村山繁雄³・ 井原康夫⁴

Neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of Amyloid β (Aβ) peptides into extracellular plaques of the brain. The Aβ peptides, composed of forty amino acids, are generated from amyloid precursor proteins (APP) by β- and γ-secretases. Aβ is deposited not only in cerebral parenchyma but also in leptomeningeal and cerebral vessel walls, known as cerebral amyloid angiopathy (CAA). While a variety of Aβ peptides were identified, detailed production and distribution of individual Aβ peptides in pathological tissues of AD and CAA is not fully addressed. Here, we develop a novel protocol of MALDI-imaging mass spectrometry (MALDI-IMS) in combination with shotgun proteomics on human autopsy brain tissues to obtain a comprehensive protein mapping.