演題概要

ポスター発表

第1日　5月15日（水）　　P会場（多目的ホール）

1P-24　　PDF

ダイレクトnanoESI-質量分析における網羅的イオン化補正法の開発

(¹静岡県大薬・ ²横河電機)
^o加藤良浩¹・ 田中奈津美¹・ 水野初¹・ 工藤忍²・ 豊岡利正¹・ 轟木堅一郎¹

Direct nanoelectrospray ionization (nanoESI) – mass spectrometry is extremely useful for a single cell analysis to detect trace amount of metabolites. However, various concomitant constituents in a cell cause matrix effects upon ionization and it hinders an accurate analysis due to fluctuations in peak intensities. The extracts of algal cells cultivated with a stable isotope labeled medium were used as an internal standard mixture and it was found useful to lessen the fluctuation. This internal standard method was applied to elucidate the influence of inorganic ions such as Na⁺, K⁺, Li⁺, Cl^-, and PO₄^3-, concomitantly present in a cell to the ionization of cellular endogenous metabolites. There found a trend between ionization tendency and matrix effects in their ionization.