ポスター発表
- 第1日 5月15日(水) P会場(多目的ホール)
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1P-07 PDF
異なる緩衝剤中での抗体の安定性と構造の関係
Introduction
It has been recently recognized that antibody aggregates may result in adverse effects such as immunogenicity and decrease in drag efficacy. Thus, formulation optimizations of antibody drugs are necessary to minimize aggregation. Mechanistic understanding of buffer contribution to the stability could pave the way for the rational formulation development.
Results
Higher aggregation propensity was observed in citrate buffer. Zeta potential of antibody molecule in citrate buffer was lower than that in acetate buffer. Secondary virial coefficient showed repulsive interaction between antibody molecules in acetate buffer while attractive interaction in citrate buffer. HDX-MS detected some regions with less conformational flexibility in citrate buffer than acetate buffer.
Discussion
Lower absolute value of zeta potential in citrate buffer suggested, upon binding of citrate ions which has higher valence number than acetate ions on antibody molecules, leading to higher shielding effect of the ions. This preferential binding of citrate ions could cause structural change on specific regions, which induce difference of interaction. Higher aggregation property in citrate buffer could be attributed to attractive interaction between antibody molecules. In conclusion, aggregation difference between acetate buffer and citrate buffer was rationally explained by biophysical parameter.