日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第1日 5月15日(水)  P会場(多目的ホール)

nanoLC-MSを用いたインタクトタンパク質の高感度分析

(1名大ITbM2信和化工)
o加納圭子1小林宏資2和田啓男2桑田啓子1

Protein identification is usually done by “bottom-up” proteomics, in which the proteins are digested by trypsin prior to LC-MS/MS analysis. However, in case of truncated protein for example, clarification of the terminal amino acid position is often difficult by “bottom-up” proteomics. Although using conventional LC-MS allows us to detect truncated proteins efficiently, it is difficult when the amount of truncated proteins is too low. Therefore, it is important to detect intact protein molecules by nanoLC-MS. In this presentation, a monolithic silica capillary column that was developed by Shinwa Chemical Industries Ltd. was connected to nanoLC-MS to evaluate the performance using co-immunoprecipitated proteins.