ポスター発表
- 第1日 5月15日(水) P会場(多目的ホール)
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1P-04 PDF
低親和性ビオチン結合タンパク質を用いたペプチド捕捉型BioID(近位依存性ビオチン標識)法による新規タンパク質間相互作用マッピング法
Biotin-based labeling strategies such as BioID (proximity-dependent Biotin IDentification) and DiDBiT (Direct Detection of Biotin-containing Tag) are widely employed to study protein-protein interactions. While the high affinity of avidin for biotin greatly facilitates the capture of biotinylated proteins, it still presents a challenge for the recovery of biotinylated peptides. In the present study, we describe a Tamavidin-based biotinylated peptide capture strategy for mapping protein-protein interactions by liquid chromatography tandem mass spectrometry (LC/MS/MS). Tamavidins found in a Tamogitake mushroom are biotin-binding protein with reversible biotin-binding capability.