日本質量分析学会 第67回質量分析総合討論会

演題概要

ポスター発表

第1日 5月15日(水)  P会場(多目的ホール)

低親和性ビオチン結合タンパク質を用いたペプチド捕捉型BioID(近位依存性ビオチン標識)法による新規タンパク質間相互作用マッピング法

(1神戸大バイオ2神戸大院医3神戸大院農4神戸薬大)
o吉野健一1,2西原萌華3上田修司3竹内敦子4

Biotin-based labeling strategies such as BioID (proximity-dependent Biotin IDentification) and DiDBiT (Direct Detection of Biotin-containing Tag) are widely employed to study protein-protein interactions. While the high affinity of avidin for biotin greatly facilitates the capture of biotinylated proteins, it still presents a challenge for the recovery of biotinylated peptides. In the present study, we describe a Tamavidin-based biotinylated peptide capture strategy for mapping protein-protein interactions by liquid chromatography tandem mass spectrometry (LC/MS/MS). Tamavidins found in a Tamogitake mushroom are biotin-binding protein with reversible biotin-binding capability.