- 第1日 5月15日（水） P会場（多目的ホール）
Biotin-based labeling strategies such as BioID (proximity-dependent Biotin IDentification) and DiDBiT (Direct Detection of Biotin-containing Tag) are widely employed to study protein-protein interactions. While the high affinity of avidin for biotin greatly facilitates the capture of biotinylated proteins, it still presents a challenge for the recovery of biotinylated peptides. In the present study, we describe a Tamavidin-based biotinylated peptide capture strategy for mapping protein-protein interactions by liquid chromatography tandem mass spectrometry (LC/MS/MS). Tamavidins found in a Tamogitake mushroom are biotin-binding protein with reversible biotin-binding capability.