演題概要

ポスター発表

第1日　5月15日（水）　　P会場（多目的ホール）

1P-03　　PDF

Salmonellaの血清型レベルのプロテオタイピングのためのMALDI-MS分析法の開発

(¹島津・ ²名城大)
^o福山裕子¹・ 加藤晃代²・ 永井里美²・ 島圭介¹・ 船津慎治¹・ 山田賢志¹・ 田村廣人²・ 野村静男¹・ 緒方是嗣¹・ 関谷禎規¹・ 岩本慎一¹・ 田中耕一¹

Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been used for rapid identification of microorganisms. Recently, a mass database consisting of twelve biomarker proteins: S8, L15, L17, L21, L25, S7, superoxide dismutase (SodA), peptidylprolyl cis-trans isomerase C, Gns, YibT, YaiA and YciF, was reported for serotyping of Salmonella enterica in MALDI-MS (Applied Microbiology and Biotechnology, 2017, 101, 8557-8569). However, it had difficulty in reproducible peak detection of the biomarkers such as SodA at m/z 23000. We report here an optimized preparation for detecting the biomarkers with high sensitivity and reproducibility. As a result, the difficulty was solved especially by controlling bacterial concentration at 6×10⁶~3×10⁷ CFU/μL, using the supernatant of the colony suspension by centrifugation, and adding matrix additives such as methylenediphosphonic acid and N-decyl-β-D-maltopyranoside in the suspension.