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- 第1日 5月15日（水） 15:45～16:05 C会場（101）
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1C-O1-1545 PDF
LC-MS/MSを用いた血清中の抗体薬物複合体（ADC）の定量 —ターゲット選定から定量系構築まで—
An analytical method for quantifying ADC in serum with LC-MS/MS has been developed using Kadcyla which is commercially available ADC. As a result of measuring an enzymatic digest of Kadcyla using trypsin, V8 and Asp-N using Q-TOF/MS, 32 of 44 sites of lysine in Kadcyla were identified as conjugation sites of DM-1. The conjugation ratio of DM-1 for each lysine was estimated from the peak area ratio of drug conjugate to non-conjugate. Based on these results, a specific payload-linked peptide was selected from the tryptic digest. By using these peptides, a bioanalytical method with LC-MS/MS was constructed.