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第1日　5月15日（水）　16:25～16:45　B会場（102）

1B-O1-1625　　PDF

Hydrogen Attachment/Abstraction Dissociation (HAD)-MS/MS及びその関連技術を用いた脂質の詳細構造分析

(¹島津・ ²同志社・ ³産総研)
^o高橋秀典¹・ 島袋祐次²・ 浅川大樹³・ 岩本慎一¹・ 和田元²・ 田中耕一¹

Tandem mass spectrometry (MS/MS) is an effective analytical technique for the characterization of structurally diverse lipids. However, distinguishing isomeric lipids using the conventional collision-induced dissociation (CID) MS/MS technique often remains ambiguities, due to its lower reproducibly and specificity. To solve this limitation, a novel MS/MS technique of hydrogen attachment/abstraction dissociation (HAD) and oxygen attachment dissociation (OAD) was developed by our research group. In this study, we demonstrated the usefulness of HAD and OAD for double-bond position analysis of lipid and characterization of trans unsaturated fatty acids.