Program

Poster Presentations

Day 4, May 18（Fri.）　　Poster

4P-27　　PDF

Effects of Memantine on the Growth and Protein Profiles of Neuroblastoma Cells

(¹Clin. Proteomics Mol. Med., St. Marianna Univ. Grad. Sch. Med., ²Adv. Med. Res., St. Marianna Univ. Grad. Sch. Med., ³Dis. Biomarker Anal. Mol. Reg., St. Marianna Univ. Grad. Sch. Med.)
^oMasaaki Sato¹, Kiyoshi Sekiguchi², Toshiyuki Sato¹, Atsuhiro Tutiya¹, Kazuki Omoteyama¹, Mitsumi Arito¹, Naoya Suematsu¹, Tomohiro Kato¹, Manae Kurokawa³

Objective: Memantine, a low-affinity NMDA receptor antagonist, is one of primary pharmacological therapies for Alzheimer's disease. To explore novel effects of memantine, we analyzed protein profile change caused by memantine using neuroblastoma cells.
Methods: Human neuroblastoma GIMEN cells were cultured in the presence or absence of 1-100 μM memantine for 48 hours. Effects of memantine on the cell growth were evaluated by counting cell numbers. Proteins were extracted from GIMEN treated with 10 μM memantine and separated by 2D-DIGE. Protein spots of interest were identified by mass spectrometry.
Results: Memantine suppressed the growth of GIMEN in a dose-dependent manner (2-10 μM, 85.5-76.1%, p<0.05; 20-100 μM, 70.0-63.0%, p<0.01). 892 protein spots were detected in the 2D-DIGE of 10 μM memantine-treated and non-treated GIMEN. 13 protein spots showed 1.2-fold or higher intensity by the memantine-treatment than no-treatment, whereas 19 protein spots showed -1.2 (1/1.2)-fold or lower intensity (p<0.05). We identified 6 proteins in 7 out of the 32 spots, which included decreased expression of β-actin, γ-enolase, and glutathione synthetase. These proteins are known to be involved in cell proliferation individually and/or in concert.
Conclusion: Memantine affected protein profiles of GIMEN. Our data suggested a novel effect of memantine to suppress the neuroblastoma cell growth, which may be associated with decreased expression of β-actin, γ-enolase, and glutathione synthetase.